Description

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產(chǎn)品英文名稱(chēng)：MesenCult?-SF Culture Kit
產(chǎn)品中文名稱(chēng)：MesenCult? SF培養試劑盒

MesenCult?-SF Culture Kit 產(chǎn)品英文描述
MesenCult?-SF Culture Kit is a standardized, serum-free system for the culture of human mesenchymal stem cells (MSCs). ?It consists of MesenCult?-XF Medium (Catalog #05420) and MesenCult?-SF Attachment Substrate (Catalog #05424) and is optimized for the expansion of MSCs in vitro as well as their enumeration using the colony-forming unit - fibroblast (CFU-F) assay. This product also supports long-term growth of MSCs and cells maintain multi-lineage differentiation potential. The MesenCult?-SF Culture Kit must be used in conjunction with the MesenCult?-ACF Dissociation Kit (Catalog #05426). Components of the MesenCult?-ACF Dissociation Kit are prescreened and tested for optimal cell adherence when cells are passaged and cultured with MesenCult?-XF Medium. MesenCult?-XF Medium must be supplemented with L-glutamine.

MesenCult? SF培養試劑盒推薦使用
? Mesenchymal stem cell expansion from primary human bone marrow
? Long-term culture of mesenchymal stem cells (greater than 8 passages)
? Detection and enumeration of CFU-F from primary human bone marrow and culture-expanded mesenchymal stem cells

MesenCult?-SF Culture Kit 產(chǎn)品組分
? MesenCult?-XF Basal Medium, 400 mL (Catalog #05421)
? MesenCult?-XF Supplement (5X), 100 mL (Catalog #05422)
? MesenCult?-SF Attachment Substrate, 5 mg (Catalog #05424)

MesenCult?-SF Culture Kit 保存方法
05421 MesenCult?-XF Basal Medium ,400 mL
Product stable at 2 - 8°C for one year from date of manufacture as indicated on label.
05422 MesenCult?-XF Supplement (5X) ,100 mL
Product stable at -20°C for one year from date of manufacture as indicated on label. Do not store at 2 - 8°C.

MesenCult? SF培養試劑盒一些相關(guān)實(shí)驗資料
Human bone marrow-derived MSCs cultured in MesenCult?-XF expand faster than cells cultured in a traditional serum-based medium (n = 3; Mean ± SEM)

Human bone marrow-derived MSCs cultured in MesenCult?-XF display multi-lineage differentiation potential

A. Oil red O staining of adipocytes generated from passage 1 MSCs previously cultured in MesenCult?-XF.B. Alizarin Red detection of Ca2+ deposits indicates the formation of bone structures in cells generated from passage 4 MSCs previously cultured in MesenCult?-XF.C. Alcian Blue staining of chondrocytes generated from passage 2 MSCs previously cultured in MesenCult?-XF.D. Collagen II staining of chondrocytes generated from passage 2 MSCs previously cultured in MesenCult?-XF.

Phenotype of culture-expanded human MSCs cultured in MesenCult?-XF
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MesenCult? SF培養試劑盒相關(guān)產(chǎn)品訂購信息
Stemcell ?05420 ?MesenCult?-XF Medium ?Defined, Xeno-Free Medium for Human Mesenchymal Stem Cells ?
Stemcell ?05424 ?MesenCult?-SF Attachment Substrate ?Serum-Free Attachment Substrate for Human Mesenchymal Stem Cells ?
Stemcell ?05426 ?MesenCult?-ACF Dissociation Kit ?Dissociation Kit for Human Mesenchymal Stem Cells ?
Stemcell ?05411 ?MesenCult? Proliferation Kit (Human) ?Medium for Detection of CFU-F and Expansion of Human Mesenchymal Stem Cells ?
Stemcell ?18659 ?EasySep? Human CD271 Selection Kit ?Immunomagnetic Positive Selection Kit ?
Stemcell ?07100 ?L-Glutamine ?L-Glutamine (200 mM) ?
For research use only. not intended for human or animal diagnostic or therapeutic uses.

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說(shuō)明書(shū)
Stemcell 05429 MesenCult? SF培養試劑盒產(chǎn)品說(shuō)明書(shū)

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