TMB（3，3'，5，5'-四甲基聯(lián)苯胺）是辣根過(guò)氧化物酶（HRP）的發(fā)色底物。 在HRP對過(guò)氧化氫進(jìn)行酶促降解過(guò)程中，TMB產(chǎn)生深藍色，添加終止溶液產(chǎn)生清晰的黃色，并在450 nm處吸收。 與OPD（鄰苯二胺二氯化物）相比，該底物可提供更高的信號并在ELISA中具有更高的靈敏度。

TMB ONE characteristics are:

• “The Gold Standard” used across the industry
• Incredibly stable and robust
• Aqueous based – no organic solvents (REACH Annex XVII compliant)
• Low lot-to-lot variation

For traceable pipetting, we recommend?using pink colored TMB ONE Prestained?(cat. no. 4430). The pink indicator disappears?after addition of the stop solution.

The product is?available in bulk quantities and as customized solutions.

Specifications：

Available sizes: 100 mL, 500 mL, 1 L , bulk? ? ? ? ? ? ? ? ? ? ? Format:Ready-to-use (RTU)
Storage: 2-8?C in the dark? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?Stability: 4 years
Development time: 5-30 minutes? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?Stop solution: 0.2 M H?SO??

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